CCNE1 and survival of patients with tubo-ovarian high-grade serous carcinoma: An Ovarian Tumor Tissue Analysis consortium study

BACKGROUND: Cyclin E1 (CCNE1) is a potential predictive marker and therapeutic target in tubo-ovarian high-grade serous carcinoma (HGSC). Smaller studies have revealed unfavorable associations for CCNE1 amplification and CCNE1 overexpression with survival, but to date no large-scale, histotype-specific validation has been performed. The hypothesis was that high-level amplification of CCNE1 and CCNE1 overexpression, as well as a combination of the two, are linked to shorter overall survival in HGSC. METHODS: Within the Ovarian Tumor Tissue Analysis consortium, amplification status and protein level in 3029 HGSC cases and mRNA expression in 2419 samples were investigated. RESULTS: High-level amplification (>8 copies by chromogenic in situ hybridization) was found in 8.6% of HGSC and overexpression (>60% with at least 5% demonstrating strong intensity by immunohistochemistry) was found in 22.4%. CCNE1 high-level amplification and overexpression both were linked to shorter overall survival in multivariate survival analysis adjusted for age and stage, with hazard stratification by study (hazard ratio [HR], 1.26; 95% CI, 1.08-1.47, p = .034, and HR, 1.18; 95% CI, 1.05-1.32, p = .015, respectively). This was also true for cases with combined high-level amplification/overexpression (HR, 1.26; 95% CI, 1.09-1.47, p = .033). CCNE1 mRNA expression was not associated with overall survival (HR, 1.00 per 1-SD increase; 95% CI, 0.94-1.06; p = .58). CCNE1 high-level amplification is mutually exclusive with the presence of germline BRCA1/2 pathogenic variants and shows an inverse association to RB1 loss. CONCLUSION: This study provides large-scale validation that CCNE1 high-level amplification is associated with shorter survival, supporting its utility as a prognostic biomarker in HGSC

SWDF and DBpedia Live Changeset used in Iguana Benchmark 2016

The DBpedia Live and SWDF generated changesets used in the Iguana Feasible SPARQL Benchmark 2016. <br

IGUANA forum

Forum: https://groups.google.com/d/forum/iguana-aksw <br>Mailing List: [email protected] <br

Queries: DBpedia

250 Benchmark queries for the DBpedia dataset<br

DBPSBv2 execution on 16 querying user

Collection of raw results, figures and gnumeric file for the figures of the execution of the DBPSBv2 queries with IGUANA on an 400M Triple Dbpedia dataset.<br><br>- results_again.zip : raw results<br>- results_dbpsb.gnumeric : gnumeric file to create the figures<br>- dbpsb_16_qmph.pdf : Figure for 16 querying users and a comparison between 0 and 1 update user (Metric: Query Mixes Per Hour)<br>- dbpsb_16_no-of-queries.pdf : Figure for 16 querying users and a comparison between 0 and 1 update user (Metric: No of queries Per Hour)<br>- dbpsb_16-1_qps.pdf : Figure for 16 querying users and 1 update user (Metric: Query Per Second)<br>- dbpsb_16-0_qps.pdf : Figure for 16 querying users and no update user (Metric: Query Per Second)<br

IGUANA Stresstest overview

Overview of the Stresstest implemented in IGUANA<br

LUBM execution on 1 mix as well as 1hour with 1 and 16 users on 20University dataset

Executing LUBM [1] Benchmark on 20 University dataset (generated with LUBM Data Generator [1]) <br><br>- Executing against virtuoso7, fuseki 2.3.0 (Jena tdb), blazegraoh 1.5.3 on standard settings<br>- Dataset has 20 Universities<br>- The 14 Queries can be found here [2] (be aware that iguana need each query in one own line)<br>- Upload took:<br>     - Blazegraph: 78580ms<br>     - Virtuoso: 10633ms<br>     - Fuseki: 31000ms<br>- Querying 1 mix as well as 1 hour<br><br>File Description:<br>- lubm_1-mix_1-user_qps.pdf : 1 Query Mix of 14 queries with 1 querying user (metrics: Queries Per Second)<br>- lubm_1-mix_16-user_qps.pdf : 1 Query Mix of 14 queries with 16 querying user (metrics: Queries Per Second) <br>- lubm_small.gnumeric : gnumeric file for the figures<br>- results_lubm_small.zip : Raw results for mix benchmark<br><br>- lubm_1-hour_16-user_qps.pdf : 1 hour of 14 queries with 16 querying users (metrics Queries Per Second)<br>- lubm_1-hour_16-user_qmph.pdf : 1 hour of 14 queries with 16 querying users (metrics Query Mixes Per Hour)<br>- lubm_1-hour_16-user_no-of-queries.pdf : 1 hour of 14 queries with 16 querying users (metrics No of Queries per Hour)<br>- lubm_hour.gnumeric : gnumeric file for hour figures<br>- results_lubm_hour.zip : Raw results for hour benchmark<br><br>-------<br>[1] http://swat.cse.lehigh.edu/projects/lubm/<br>[2] http://swat.cse.lehigh.edu/projects/lubm/queries-sparql.txt <br

Warmup: DBpedia

Warmup Queries for the DBpedia dataset<br